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KMID : 0848120060310010015
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International Journal of Oral Biology
2006 Volume.31 No. 1 p.15 ~ p.20
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Mode of Cell Death and Molecualar Change of Oral Squamous Carcinoma Cells Exposed to Metal Ions
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Kim Hyung-Il
Baek Chang-Jun
Kim In-Ryoung
Kim Hyung-Keun
Park Hae-Ryoun
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Abstract
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Concerns remain regarding the biocompatibility and adverse effects of dental casting alloys. The aim of this study was to understand the cytopathogenic effect of metal ions, which might be released from dental alloys, on oral squamous carcinoma(OSC) cells. The cellular morphology, viability, the type of cell death and molecular change in response to metal ion salt solutions including aluminum(Al), cobalt(Co), copper(Cu) and nickel(Ni) were examined. The TC50 values for the metal ions with the exception of AI were estimated to be between 400 and 600μM. The cells treated with the metal ions showed apoptotic change with the exception of Al ions. Metal ion-induced apoptosis was further confirmed using flow cytometric analysis. This study showed that the cytotoxicity and the mode of cell death by metal ions clearly depend on the cell type, the type of metal ion and the duration of exposure. The protein level of Rb, a tumor suppressor that affects apoptosis para-doxically, was higher in the cells treated with Co, Cu and Ni. It is believed that apoptosis and cell damage in the OSC cells treated with Co, Cu or Ni can be evoked by the regulation of Rb.
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KEYWORD
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dental alloys, metal ions, oral squamous carcinoma cells, apoptosis
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